Overexpression of a Thioredoxin-Protein-Encoding Gene, MsTRX, from Medicago sativa Enhances Salt Tolerance to Transgenic Tobacco

Thioredoxin (TRX) is a small molecule protein that participates in the redox process and plays decisive role various functions of plants. However, TRX Medicago sativa (alfalfa), widely cultivated perennial herb legume, still poorly understood. Here, we isolated MsTRX from alfalfa determined characteristics improving salt tolerance by assaying phenotype physiological changes expression stress-response genes transgenic tobacco. The was similar roots, leaves, inflorescences, downregulated response to cold, drought, treatment. overexpression tobacco promoted accumulation soluble sugar (SS) proline; enhanced activity peroxidase (POD); induced upregulation beta-amylase 1 (BAM1), lipid-transfer (LTP1), candidate signal molecules/sensor relay proteins (CBSX3), superoxide dismutase [Cu-Zn] (Cu/Zn-SOD), [Mn] (Mn-SOD), gamma (GR1), dehydrin DHN1-like (ERD10B), serine/threonine-protein kinase (SnRK2), as well downregulation phyB activation-tagged suppressor1 (BAS1) phosphorylates LHCII 7 (STN7) under stress. These results indicated improves via maintaining osmotic homeostasis, scavenging reactive oxygen species (ROS), regulating transcription In our study, provided new understanding how stress plants can be included future breeding programs improve alfalfa.